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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide normally contains the epitope acknowledged with the antibody.
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This tutorial outlines more possible optimization of mobile and immunoassay parameters to ensure the very best success are...
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This tutorial outlines more attainable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...
Antibodies bound to the blocking peptide no longer bind on the epitope over the concentrate on protein. By comparing the staining in the blocked antibody vs . the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development via S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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